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Effect of high temperature on growth, nitrogen fixation, and chlorophyll content of five species of Azolla Anabaena symbiosis

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Summary

Cultures of Azolla sp. ST-SI, A. microphylla BR-GI, A. mexicana BR-GL, A. caroliniana WT-V, and A. filiculoides BR -H were grown in N-free International Rice Research Institute growth medium in the glasshouse at 38±1 °C (day) and 25±1 °C (night) under a light intensity of 350 μEm2s−1 for 27 days. Biomass, chlorophyll contents and nitrogenase activity (acetylene reduction assay) were recorded on the 19th and 27th day. For comparison the same parameters were studied in Azolla spp. under normal growth conditions at 26±1 °C (day) and 19±1 °C (night). Azolla sp. STSI and A. microphylla BR-GI had produced a larger biomass by the 19th and the 27th day of incubation than A. caroliniana WTV and A. filiculoides which showed poor growth. Under normal growth conditions A. caroliniana WTV and A. filiculoides BRH produced less biomass than the other Azolla spp. cultures tested. A. mexicana BR-GL had a higher total chlorophyll content in both incubation periods than A. caroliniana WT-V and A. filiculoides BR-H. The N content was high in Azolla sp. ST-SI, A. microphylla BR-GI, and A. mexicana BR-GL compared with the low N content of A. filiculoides BR-H and A. caroliniana WT-V. At the higher temperature (38±1 °C/25±1 °C) Azolla sp. ST-SI and A. microphylla BR-GI consistently showed a higher growth rate than A. filiculoides BR-H and A. caroliniana WTV, while the growth rate of A. mexicana BR-GL was intermediate.

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References

  • Becking JH (1979) Environmental requirements of Azolla for use in tropical rice production. In: Nitrogen and rice. Int Rice Res Inst (Los Baños), pp 345–373

  • FAO (1977) China: Recycling of organic wastes in agriculture. FAO Soils Bull 40:29–38

    Google Scholar 

  • Kannaiyan S (1982) Azollae and rice. In: Multiplication and use of Azolla biofertilizer for rice production training. Tamil Nadu Agric Univ Coimbatore, Tamil Nadu, India, p 58

    Google Scholar 

  • Kannaiyan S, Thangaraju M, Oblisami G (1982) Influence of Azolla biofertilizer application as green manure and dual cropping on rice. Natl Sci Acad Newslett 5:60–62

    Google Scholar 

  • Peters GA, Mayne BC (1974) Azolla-Anabaena azollae relationship: II. Localization of nitrogenase activity as assayed by acetylene reduction. Plant Physiol 53:820–824

    Google Scholar 

  • Peters GA, Toia RE Jr, Evans WR, Crist DK, Mayne BC, Poole RE (1980) Characterization and comparisons of five N2 fixing Azolla Anabaena associations: I. Optimization of growth conditions for biomass and N content in a controlled environment. Plant Cell Environ 3:261–269

    Google Scholar 

  • Ray TB, Peters GA, Toia RE Jr, Mayne BC (1978) The Azolla-Anabaena azollae relationship: VII. Distribution of ammonia assimilating enzymes, protein and chlorophyll between host and symbiont. Plant Physiol 62:463–467

    Google Scholar 

  • Subudhi BPR, Watanabe I (1981) Differential phosphorus requirements of Azolla species and strains in phosphorus limited continuous culture. Soil Sci Plant Nutr 27:237–247

    Google Scholar 

  • Tung HF, Watanabe I (1983) Differential response of Azolla Anabaena associations to high temperature and minus phosphorus treatments. New Phytol 93:423–431

    Google Scholar 

  • Tyagi UVS, Mayne BC, Peters GA (1980) Purification and initial characterization of phycobiliproteins from the endophytic cyanobacterium of Azolla. Arch Microbiol 128:41–44

    Google Scholar 

  • Watanabe I (1982) Azolla-Anabaena symbiosis — its physiology and use in tropical agriculture. In: Dommergues Y, Diem H (eds) Microbiology of tropical soils and productivity. Martinus Nijhoff, The Hague, pp 169–185

    Google Scholar 

  • Watanabe I, Berja NS (1983) The growth of four species of Azolla as affected by temperature, Aquatic Bot 15:175–185

    Google Scholar 

  • Watanabe I, Espinas CR, Berja NS, Alimagno BA (1977) Utilization of the Azolla-Anabaena complex as a nitrogen fertilizer for rice. Int Rice Res Inst (Los Baños) Res Pap Ser 11

  • Wintermans JFGM, Demots A (1965) Spectrophotometric characteristics of chlorophyll a and b and their pheophytins in ethanol. Biochem Biophys Acta 109:448–453

    Google Scholar 

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The study was carried out at C.F. Kettering Research Laboratory, Yellowsprings, OH - 45387, USA

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Kannaiyan, S., Somporn, C. Effect of high temperature on growth, nitrogen fixation, and chlorophyll content of five species of Azolla Anabaena symbiosis. Biol Fert Soils 7, 168–172 (1989). https://doi.org/10.1007/BF00292577

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  • DOI: https://doi.org/10.1007/BF00292577

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